FIFTH SEMESTER CHEMISTRY
Analytical Methods in Chemistry-2-7B
Unit-1
Chromatography-Introduction and classification :Principle, Classification of chromatographic methods, Nature of adsorbents, eluents, Rf values, factors affecting Rf values.
UNIT-2
TLC and paper chromatography
1. Thin layer chromatography: Principle, Experimental procedure, preparation of plates, adsorbents and solvents, development of chromatogram, detection of spots, applications and advantages.
2. Paper Chromatography: Principle, Experimental procedure, choice of paper and solvents, various modes of development- ascending, descending, radial and two dimensional, applications.
UNIT-3
Column chromatography
1. Column chromatography: Principle, classification, Experimental procedure, stationary and mobile phases, development of the Chromatogram, applications.
2. HPLC: Basic principles, instrumentation –block diagram and applications.
UNIT-4
Principle, Instrumentation: Single beam and double beam spectrometer, BeerLambert’s law- Derivation and deviations from Beer-Lambert’s law, applications of BeerLambert’s law-Quantitative determination ofFe+2, Mn+2and Pb+2.
UNIT-5:
Atomic spectroscopy :Types, atomizer, atomic absorption and emission and applications.